动物大脑中动脉栓塞（middle cerebral artery occlusion, MCAO）模型是目前使用最为广泛的、研究局灶性脑缺血再灌注损伤的理想模型。线栓法是制作MCAO模型常用方法，MCAO线栓（MCAO Monofilaments, MCAO Sutures）则是制备大鼠、小鼠（或其它实验动物）这一模型非常关键的实验材料，本产品采用柔韧度非常好的进口单丝尼龙线，经显微操作，头端均匀包被硅胶，其表面光滑，粗细一致，易进入颅内又不至于刺破血管，使用本产品可大大提高模型制备的成功率，及脑缺血范围的稳定性。

 
对于MCAO模型来说，主要有两种线拴，无包被的火焰熔烫圆头线拴及硅胶包被线拴。研究表明(Tsuchiya et al. 2003)，无包被的圆头线拴在MCAO实验过程中可能导致高达40%的蛛网膜下腔出血，而其标准差可能比平均值的50%还要大。另一项技术文章表明(Shimamura et al. 2006a)，硅胶包被线拴要远好于无包被的圆头线拴，其结果更为稳定，即使对于没有经验的实验者来说也是如此。而多聚赖氨酸包被的线拴只能增加死亡率，不能减少梗赛体积的变异程度(Huang et al. 1998)。



Doccol MCAO线拴全部为硅胶包被，圆润、柔软、包被均匀，即使在没有激光多普勒实时检测的情况，也不会将血管戳破。使用自制的硅胶包被的线拴，大鼠(Schmid-Elsaesser et al. 1998)及小鼠(Shah et al. 2006)的脑梗塞体积的标准差均在30%左右。而使用Doccol MCAO线拴可以得到更好的结果。对于大鼠MCAO模型来说，使用Doccol MCAO线拴得到的结果的标准差可达10%-20% （Ruscher et al.2012；Ishizaka et al.2013；Sakata et al.2012；Candelario-Jalil et al. 2008；Khan et al. 2006；Liu et al. 2006；Shimamura et al. 2006b；Solaroglu et al. 2006；Tsubokawa et al. 2007；Tsubokawa et al. 2006a；Tsubokawa et al. 2006b)。对于小鼠来说，使用Doccol MCAO线拴得到的结果的标准差可达5%-10%(Chen et al.2014; Bae et al.2013; Jin et al.2011; Gu et al.2012; Kleinschnitz et al. 2007; Maysami et al. 2008; Pignataro et al. 2007b; Pignataro et al. 2007c)。





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