分子量:37.1 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Mouse Dkk-3, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:

产品特性:Mouse Dkk-3, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 37.1 kDa. The predicted N-terminus is Pro 23. The reducing (R) protein migrates as 55-66 kDa in SDS-PAGE  due to glycosylation.

产品背景:Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK3 has been proposed as tumour suppressor gene and a marker for tumour blood vessels. DKK3 is the only DKK family member abundantly expressed in normal lung, but silenced by promoter hypermethylation in a large fraction of lung cancer cell lines and lung tumors. Downregulation of DKK3 was correlated with tumor progression and expression of nuclear beta-catenin in lung tumors. Ectopic expression of DKK3 in lung cancer cells with DKK3 hypermethylation induced apoptosis and inhibited TCF-4 activity as well as nuclear accumulation of beta-catenin and expression of TCF-4 targets c-Myc and cyclin D1. DKK3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development, was reported.