分子量:17.7 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Fas Ligand, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.1-1.5 ng/mL in the presence of 10 μg/mL of a crosslinking antibody Mouse Anti poly-Histidine Monoclonal Antibody.

产品特性:Human Fas Ligand, His Tag is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 17.7 kDa. The predicted N-terminus is Pro 134 . The reducing (R) protein migrates as 25-32 kDa in SDS-PAGE  due to glycosylation.

产品背景:Fas ligand is also known as FasL, CD178, CD95L, or TNFSF6, is a homotrimeric type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Mature human Fas Ligand consists of a 179 amino acid (aa) extracellular domain (ECD), a 22 aa transmembrane segment, and a 80 aa cytoplasmic domain. Within the ECD, human Fas Ligand shares 81% and 78% aa sequence identity with mouse and rat Fas Ligand, respectively. Apoptosis triggered by Fas-Fas ligand binding plays a fundamental role in the regulation of the immune system. Its functions include:T-cell homeostasis, cytotoxic T-cell activity, immune privilege, maternal tolerance, tumor counterattack. Defective Fas-mediated apoptosis may lead to oncogenesis as well as drug resistance in existing tumors. Germline mutation of Fas is associated with autoimmune lymphoproliferative syndrome (ALPS), a childhood disorder of apoptosis.