分子量:25.8 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human IgG4 Fc, Tag Free by the LAL method.

Buffer:50 mM tris, 100 mM glycine, pH7.5

生物活性:

产品特性:Human IgG4 Fc, Tag Free contains no "tag", and has a calculated MW of 25.8 kDa. The predicted N-terminus is Glu 99. As a result of glycosylation, the reducing (R) protein migrates as 33-35 kDa, and the non-reducing (NR) protein migrates as 50-55 kDa in SDS-PAGE .

产品背景:Immunoglobulin G4 (IgG4) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-4 chain Fc region contains two constant regions of IgG4 H chain (CH2, CH3).