USP18 polyclonal antibody
产品名称： USP18 polyclonal antibody
英文名称： USP18 polyclonal antibody
产品编号： PAB10241
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of USP18.
- Immunogen:
- A synthetic peptide corresponding to amino acids 40-49 of human USP18.
- Host:
- Rabbit
- Reactivity:
- Chimpanzee, Dog, Human, Mouse, Orangutan
- Specificity:
- This affinity-purified antibody is directed against human UBP43 protein.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:10000-1:50000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- The ISG15 isopeptidase UBP43 is regulated by proteolysis via the SCFSkp2 ubiquitin ligase.
Tokarz S, Berset C, La Rue J, Friedman K, Nakayama K, Nakayama K, Zhang DE, Lanker S.J Biol Chem. 2004 Nov 5;279(45):46424-30. Epub 2004 Sep 1.
- 2.
- Human and mouse proteases: a comparative genomic approach.
Puente XS, Sanchez LM, Overall CM, Lopez-Otin C.Nat Rev Genet. 2003 Jul;4(7):544-58.
- 3.
- Cloning and characterization of human ubiquitin-processing protease-43 from terminally differentiated human melanoma cells using a rapid subtraction hybridization protocol RaSH.
Kang D, Jiang H, Wu Q, Pestka S, Fisher PB.Gene. 2001 Apr 18;267(2):233-42.
- 4.
- Cloning and characterization of a novel human ubiquitin-specific protease, a homologue of murine UBP43 (Usp18).
Schwer H, Liu LQ, Zhou L, Little MT, Pan Z, Hetherington CJ, Zhang DE.Genomics. 2000 Apr 1;65(1):44-52.
- 5.
- RNase-L-dependent destabilization of interferon-induced mRNAs. A role for the 2-5A system in attenuation of the interferon response.
Li XL, Blackford JA, Judge CS, Liu M, Xiao W, Kalvakolanu DV, Hassel BA.J Biol Chem. 2000 Mar 24;275(12):8880-8.
- Applications
- Western Blot (Cell lysate)
- Western blot using USP18 polyclonal antibody (Cat # PAB10241) shows detection of a band ~43 KDa band (arrowhead) believed to be to USP18 in lysates from HeLa nuclear extracts (Lane 1) and whole cell lysates from HeLa (Lane 2), A-431 (Lane 3), Jurkat (Lane 4) and HEK293 (Lane 5).
Approximately 18 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of USP18 polyclonal antibody.
Signal was detected using standard techniques.
The strong band between the 37 KDa and 50 KDa markers is expected to be USP18.
The other bands are probably non-specific.
- Entrez GeneID:
- 11274
- Protein Accession#:
- Q9UMW8;NP_059110
- Gene Name:
- USP18
- Gene Alias:
- ISG43,UBP43
- Gene Description:
- ubiquitin specific peptidase 18
- Omim ID:
- 607057
- Gene Ontology:
- Hyperlink
- Gene Summary:
- USP18, a member of the deubiquitinating protease family of enzymes, removes ubiquitin adducts from a broad range of protein substrates.[supplied by OMIM
- Other Designations:
- ubiquitin specific protease 18