分子量:21.9 kDa

纯度:>90% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human UBE2F, His Tag by the LAL method.

Buffer:PBS, pH 7.4

生物活性:

产品特性:Human UBE2F, His Tag is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 21.9 kDa. The predicted N-terminus is Met. The reducing (R) protein migrates as 23-25 kDa in SDS-PAGE .

产品背景:Ubiquitin-conjugating enzyme E2 F (UBE2F), a member of the ubiquitin-conjugating enzyme family and UBE2F subfamily, is also known as NEDD8-conjugating enzyme UBE2F, NEDD8 carrier protein UBE2F and NEDD8-conjugating enzyme 2 (NCE2),which can interact with UBA3 and RBX2. The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.UBE2F also can accept the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyze its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.